Journal: Thrombosis Research

Article Title: Recombinant ADAMTS13 reduces abnormally up-regulated von Willebrand factor in plasma from patients with severe COVID-19

doi: 10.1016/j.thromres.2021.02.012

Figure Lengend Snippet: Example electrophoretic determination of multimer size in plasma from patient B26. The plasma sample from the patient was adjusted to 1 IU/ mL VWF:Ag and separated on a 1% agarose gel followed by immunostaining and densitometry. The distance between the top of the separation well and the lowest multimer band (VWF dimers) was assigned a migration value of 1.0. In this example the relative migration distance (Rf) of the largest VWF multimer was 0.192 that of the lowest multimer band. The proportion of the total migration distance of the VWF dimer band that is occupied by larger VWF multimers is therefore 1-Rf, in this example calculated as 1.000–0.192 = 0.808. The 1-Rf value for normal plasma separated on the same gel was 0.729 (not shown). Therefore, the proportion of the patient sample lane containing VWF multimers (UHMW multimer quantitation parameter) is 111% (0.808/0.729*100 = 111%) that of control reflecting the UHMW multimers near the top of the lane.

Article Snippet: Multimer patterns were evaluated visually and quantified by densitometry (GS-900, BioRad, CA, USA).

Techniques: Clinical Proteomics, Agarose Gel Electrophoresis, Immunostaining, Migration, Quantitation Assay, Control